Application of Trichoderma Harzianum as Soil Treatment and Additional Treatment for Control of Potato Diseases
This study aims to determine the effect of biofungicide application with Trichoderma harzianum as its active ingredient in the form of soil treatment and additional treatment which includes seed treatment, canopy surface spraying, and combined seed treatment and canopy spraying with Trichoderma biofungicides and active chemical fungicides mancozeb and cymoxanil, against leaf blight disease index caused by Phytopthora infestans, stover dry weight, healthy potato tuber weight, rotten tuber weight, and relative quality index of potato plant bulbs. Experiments were carried out in the Split-plot Design using a completely randomized design (CRD). The main plot is soil tretament biofungicide Trichoderma, including without soil tretament and with soil treatment. As plot saplings is an additional treatement of Trichoderma biofungicide, consisting of: chemical fungicide, seed treatement, canopy spraying, and spraying of canopy and seed treament. Each treatment was repeated 4 times, to obtain 32 experimental units. The variables observed were leaf blight disease index at the end of the vegetative phase, stover dry weight, healthy tuber weight, rotten tuber weight, and relative index of tuber quality. The results showed that the interaction of soil treatment and additional treatment of Trichoderma biofungicide had a very significant effect on the disease index of potato leaf blight, rotten tuber weight per plant, and relative index of quality of potato tubers, but did not significantly affect the dry weight of stover and tubers of healthy potatoes per plant. The combination of soil treatment and additional treatment resulted in a decrease in the disease index of 45.37 to 53.96%, a decrease in rotten tubers from 42.39 to 91.50%, and an increase in the percentage of relative index of tuber quality from 7.8 to 65.5% compared to only using fungicides made from mancozeb and cymoxanil.
Ali, H. Z., Mohammedand, R. S., & Aboud, H. M. (2015). Efficiency of Organic Matter Levels and Bio Fungus TrichodermaHarzianumoncucumber Plant. IOSR Journal of Agriculture and Veterinary Science Ver. I, 8(6), 2319–2372. https://doi.org/10.9790/2380-08612834
Benítez, T., Rincón, A. M., Limón, M. C., & Codón, A. C. (2004). Biocontrol mechanisms of Trichoderma strains, 249–260.
Buysens, C., César, V., Ferrais, F., Dupré de Boulois, H., & Declerck, S. (2016). Inoculation of Medicago sativa cover crop with Rhizophagus irregularis and Trichoderma harzianum increases the yield of subsequently-grown potato under low nutrient conditions. Applied Soil Ecology, 105, 137–143. https://doi.org/https://doi.org/10.1016/j.apsoil.2016.04.011
Chowdappa, P., Mohan Kumar, S. P., Jyothi Lakshmi, M., & Upreti, K. K. (2013). Growth stimulation and induction of systemic resistance in tomato against early and late blight by Bacillus subtilis OTPB1 or Trichoderma harzianum OTPB3. Biological Control, 65(1), 109–117. https://doi.org/https://doi.org/10.1016/j.biocontrol.2012.11.009
Evenhuis, A., Schepers, H. T. A. M., Bus, C. B., & Stegeman, W. (1996). Synergy of cymoxanil and mancozeb when used to control potato late blight. Potato Research, 39, 551–559.
Glare, T., Caradus, J., Gelernter, W., Jackson, T., Keyhani, N., Köhl, J., … Stewart, A. (2012). Have biopesticides come of age? Trends in Biotechnology, 30(5), 250–258. https://doi.org/https://doi.org/10.1016/j.tibtech.2012.01.003
Gravel, V., Antoun, H., & Tweddell, R. J. (2007). Growth stimulation and fruit yield improvement of greenhouse tomato plants by inoculation with Pseudomonas putida or Trichoderma atroviride: Possible role of indole acetic acid (IAA). Soil Biology and Biochemistry, 39(8), 1968–1977. https://doi.org/https://doi.org/10.1016/j.soilbio.2007.02.015
Guan, C. Y. (2011). The Development Direction of The Oilseed Rape Industry in China. Grain Science Technology Economy, 36, 5–6.
Harman, G. (2006). Overview of Mechanisms and Uses of Trichoderma spp. Phytopathology (Vol. 96). https://doi.org/10.1094/PHYTO-96-0190
Harman, G., R Howell, C., Viterbo, A., Chet, I., & Lorito, M. (2004). Trichoderma species - Opportunistic, avirulent plant symbionts. Nature reviews. Microbiology (Vol. 2). https://doi.org/10.1038/nrmicro797
Howell, C. R. (2003). Mechanisms Employed by Trichoderma Species in the Biological Control of Plant Diseases: The History and Evolution of Current Concepts. Plant Disease, 87(1), 4–10. https://doi.org/10.1094/PDIS.2003.87.1.4
Hu, X., Roberts, D. P., Xie, L., Maul, J. E., Yu, C., Li, Y., … Liao, X. (2015). Components of a Rice-Oilseed Rape Production System Augmented with Trichoderma sp. Tri-1 Control Sclerotinia sclerotiorum on Oilseed Rape. Phytopathology, 105(10), 1325–1333. https://doi.org/10.1094/PHYTO-12-14-0371-R
Hu, X., Roberts, D. P., Xie, L., Yu, C., Li, Y., Qin, L., … Liao, X. (2016). Use of formulated Trichoderma sp. Tri-1 in combination with reduced rates of chemical pesticide for control of Sclerotinia sclerotiorium on oilseed rape. Crop Protection, 79, 124–127. https://doi.org/https://doi.org/10.1016/j.cropro.2015.10.020
Ma, H.-X., Feng, X.-J., Chen, Y., Chen, C., & Zhou, M. (2009). Occurrence and Characterization of Dimethachlon Insensitivity in Sclerotinia sclerotiorum in Jiangsu Province of China. Plant Disease - PLANT DIS (Vol. 93). https://doi.org/10.1094/PDIS-93-1-0036
Paramita, N. R., & Sumardiyono, C. (2014). Chemical control and resistance of Colletotrichum spp. against cymoxanil fungicide on red pepper, 18(1), 41–46.
Pruksakorn, P., Arai, M., Kotoku, N., Vilcheze, C., Baughn, A., Moodley, P., … Kobayashi, M. (2010). Trichoderins, novel aminolipopeptides from a marine sponge-derived Trichoderma sp., are active against dormant mycobacteria. Bioorganic & medicinal chemistry letters (Vol. 20). https://doi.org/10.1016/j.bmcl.2010.04.100
Saravanakumar, K., Yu, C., Dou, K., Wang, M., Li, Y., & Chen, J. (2016). Synergistic effect of Trichoderma-derived antifungal metabolites and cell wall degrading enzymes on enhanced biocontrol of Fusarium oxysporum f. sp. cucumerinum. Biological Control, 94, 37–46. https://doi.org/https://doi.org/10.1016/j.biocontrol.2015.12.001
Sutarman. (2017). Pengujian Trichoderma sp. sebagai pengendali hawar daun bibit kako yang disebabkan oleh Phytopthora palmivora. Jurnal Hama dan Penyakit Tumbuhan Tropika, 17(1), 45–52.
Vargas Gil, S., Pastor, S., & March, G. J. (2009). Quantitative isolation of biocontrol agents Trichoderma spp., Gliocladium spp. and actinomycetes from soil with culture media. Microbiological Research, 164(2), 196–205. https://doi.org/https://doi.org/10.1016/j.micres.2006.11.022
Verma, M., Brar, S. K., Tyagi, R. D., Surampalli, R. Y., & Valéro, J. R. (2007). Antagonistic fungi, Trichoderma spp.: Panoply of biological control. Biochemical Engineering Journal, 37(1), 1–20. https://doi.org/https://doi.org/10.1016/j.bej.2007.05.012
Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Barbetti, M. J., Li, H., … Lorito, M. (2008). A novel role for Trichoderma secondary metabolites in the interactions with plants. Physiological and Molecular Plant Pathology, 72(1), 80–86. https://doi.org/https://doi.org/10.1016/j.pmpp.2008.05.005
Youssef, S., Tartoura, K., & A. Abdelraouf, G. (2016). Evaluation of Trichoderma harzianum and Serratia proteamaculans effect on disease suppression, stimulation of ROS-scavenging enzymes and improving tomato growth infected by Rhizoctonia solani. Biological Control (Vol. 100). https://doi.org/10.1016/j.biocontrol.2016.06.001
Zhang, M., Liu, J.-M., Zhao, J.-L., Li, N., Chen, R.-D., Xie, K.-B., … Dai, J.-G. (2016). Two new diterpenoids from the endophytic fungus Trichoderma sp. Xy24 isolated from mangrove plant Xylocarpus granatum. Chinese Chemical Letters, 27(6), 957–960. https://doi.org/https://doi.org/10.1016/j.cclet.2016.02.008
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